Pleurotus Species as source of nutraceuticals including vitamin B12 and lignocellulosic degradative enzyme

  • Yashvant Patel Department of Biotechnology, VBS Purvanchal University, Jaunpur, India
  • Anand Prakash Department of Zoology, Sardar Vallabhbhai Patel College, Kaimur, India
  • Ankit Kumar Patel National Institute of Biomedical Genomics, Kalyani, India
  • Sanjay Kumar Vishwakarma Department of Biotechnology, Mohammad Hasan P. G. College, Jaunpur, India
Keywords: bromophenol blue, genetic diversity, malachite green, Pleurotus species, vitamin B12

Abstract

In the present study, we collected Pleurotus isolates from diverse edaphic zones from the parts of Uttar Pradesh. Neutraceuticals (proteins, carbohydrates, phenolic, vitamin B12) and xenobiotics degradation capacity of textile dyes along with the production of laccase enzyme evaluated. Isolate no. 06 appeared most distant in the dendrogram showing the highest MG degradation capacity, however, others showed excellent degradation of BPB. The laccase enzyme activity was found in the range of 4.03 to 19.13 IU/ml from mycelia extract. Gene frequency within isolates from 0.012 to 0.987 was analyzed through RAPD and the average gene diversity for all loci was 0.244 and the Shannon Information Index was found 0.397. The unbiased genetic similarity among isolates was 0.36 to 0.93 with a mean of 0.64. Significant genetic diversity, nutraceuticals and laccase enzyme availability and dye degradation capacity of the studied genus Pleurotus, was found, which makes it necessary to carry out a selection process in each one for superior selection not only for human being but also many aquatic as well as other terrestrial flora and fauna. The present investigation first time reported that Pleurotus species as a source of vitamin B12 in range of 0.05 to 0.32 mg/kg (of dried mushroom).

Author Biographies

Yashvant Patel, Department of Biotechnology, VBS Purvanchal University, Jaunpur, India

Post doctoral fellow at Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, India-221 005

Anand Prakash, Department of Zoology, Sardar Vallabhbhai Patel College, Kaimur, India

Assistant professor of Zoology, Sardar Vallabhbhai Patel College, Kaimur, India-821 101

Ankit Kumar Patel, National Institute of Biomedical Genomics, Kalyani, India

Researcher at National Institute of Biomedical Genomics, Kalyani, India-741 251

Sanjay Kumar Vishwakarma, Department of Biotechnology, Mohammad Hasan P. G. College, Jaunpur, India

Assistant professor, 

Department of Biotechnology,

Mohammad Hassan P. G. College,

Jaunpur, India-222 001

Published
2020-07-01
Section
Microbiology, Virology and Immunology