Evaluation the effects of Staphylococcus aureus Enterotoxin B on BAX, p53, Caspase3 and Bcl-2 genes expression in gastric cancer cell line

  • Matineh Rahmani Ghaleh Shahrokhi Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
  • Abbas Doosti Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Keywords: Gastric cancer, enterotoxin B, p53, caspase3, BAX, Bcl-2, RT-qPCR

Abstract

Gastric cancer is known as the fourth cancer in the world and the second cause of cancer death. According to studies, Staphylococcus aureus (S. aureus) Enterotoxin B has a major role in inducing apoptosis in various cancers. The aim of this study was to evaluate the apoptosis gene expression after gastric cancer cell treatment with enterotoxin B.

In this experimental study, the pcDNA3.1(+)–seb recombinant plasmid, after amplification in E. coli strain TOP10F and extraction was introduced into AGS cells by lipofection method. After 10 days of treatment with neomycin antibiotic, total cellular RNA was extracted and cDNA was constructed for real time PCR for apoptotic genes of p53, BAX, caspase3 and Bcl-2, as well as the GAPDH as a reference gene.

The results showed an increase in the expression of BAX, and p53 genes and decreased Bcl-2 and caspase3 expression at a significant level. As compared to the AGS cell, which did not receive the seb gene, the cells containing the toxin gene had progressed more towards apoptosis.

Enterotoxin B, expressed in gastric cancer cells, increases the expression of pro-apoptosis genes and reduces the expression of anti-apoptotic genes. According to evidence, this poison can act as an anticancer agent in the AGS cell line.

Published
2020-04-27
Section
Biochemistry and Biotechnology