Cytotoxicity of flavonoid glycosides, flavonoids and phenolic acids from Inula oculus-christi on mammalian cell lines

  • Ralitsa Krumcheva Veleva Faculty of Biology, Sofia University “St. Kliment Ohridski”, 1164 Sofia, Bulgaria
  • Veselina Moskova-Doumanova Faculty of Biology, Sofia University “St. Kliment Ohridski”
  • Milka Todorova Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria
  • Antoaneta Trendafilova Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria
  • Jordan Doumanov Faculty of Biology, Sofia University “St. Kliment Ohridski”, 1164 Sofia, Bulgaria
  • Tanya Topouzova-Hristova Faculty of Biology, Sofia University “St. Kliment Ohridski”

Abstract

Herbs of the genus Inula are well known in traditional medicine. Their extracts are used as expectorants, antitussives, bactericides as well as for the treatment of lung inflammation and have shown to possess anti-inflammatory and secretolytic activity. Experimental research findings indicate the anti-tumor effect of certain components of extracts from Inula cappa and Inula britannicа and those from Inula racemosa have antimicrobial and antidiabetic activity. We have directed our efforts on investigating the effects of different extracts from Inula oculus-christi, enriched with certain groups of biologically active substances - flavonoid glycosides (A), phenolic acids (B), flavonoid glycosides and phenolic acids (C), flavonoids and phenolic acids (D). The effect of these extracts on normal (MDCK ІІ and RPE1) and carcinoma cell lines (A549 and HepG2) was evaluated. We have performed cytotoxicity study (crystal violet assay) as well as morphological analysis of changes induced by the extracts. Among the tumor cell lines HepG2 show greater sensitivity. Surprisingly extract C has no significant influence on both cancer cell lines.
Published
2017-07-11
Section
Cellular and Molecular Biology