Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreum

Authors

  • Shobha Mehra Department of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, India
  • Ufondu Maryann Afoma Department of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, India
  • Namrata Dudha Department of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, India
  • Shilpy Singh Department of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, India
  • Shweta Sharma Department of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, India
  • Ved Vrat Verma Department of Biotechnology, School of Engineering and Technology, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, India
  • Mohammad Zuhaib Department of Agriculture, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, India
  • Varun Kumar Sharma Department of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, India

Keywords:

Phytochemical, secondary metabolite, Rhodendron arboreum, HPLC, antioxidant activity

Abstract

Phytochemicals have been known to suppress the microbial activity and oxidative stress, which indicates the significance of plant material as anti-microbial and antioxidant agent. In this light the current study is worked out to screen the phytochemicals from the Rhododendron arboretum leaf extract and characterize it using HPLC. The alkaloids and flavonoids are the major secondary metabolites found during screening. Further, anti-oxidant properties of leaf extract of R. arboreum prepared in different solvent (methanol, ethyl acetate, petroleum ether, and chloroform) were analyzed using DPPH and FRAP assay. Observation of the present study indicates that methanolic extracts was found to show significant anti-oxidant activity with the IC50 value of 241.2 µg/ml and 163.6 µg/ml in DPPH and FRAP assay respectively. In conclusion, the leaf extracts of R. arboretum can be one of the better natural source of antioxidant source and could be used for therapeutic purpose in the treatments of different diseases.

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Published

2025-01-10

How to Cite

[1]
Mehra, S. , Afoma, U.M. , Dudha, N. , Singh, S. , Sharma, S., Verma, V.V. , Zuhaib, M. and Sharma, V.K. 2025. Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreum. Journal of BioScience and Biotechnology. 13, 2 (Jan. 2025), 147–153.

Issue

Vol. 13 No. 2 (2024)

Section

Biochemistry and Biotechnology

License

Copyright (c) 2024 Shobha Mehra, Ufondu Maryann Afoma, Namrata Dudha, Shilpy Singh, Shweta Sharma, Ved Vrat Verma, Mohammad Zuhaib, Varun Kumar Sharma

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