Exploring the impact of hesperidin on probiotic properties of Lactobacillus acidophilus LA-5 and its synbiotic interaction with colon cancer cells

Abstract

Probiotics are live microorganisms that provide health benefits to the host when taken in adequate amounts. Hesperidin is a phenolic compound found in banana fruit, lemon fruit, and lemon peel. It exhibits anti-inflammatory, anti-oxidative, and anti-cancer activities. Although these compounds are present in the gastrointestinal tract (GIT), their synbiotic interaction on the GIT is not well understood. This study aims to investigate the effects of hesperidin on the probiotic properties of Lactobacillus acidophilus LA-5 (LA-5) and the impact of their synbiotic interaction on the cancer cell line HT-29. Results showed that hesperidin did not negatively affect the auto-aggregation, adhesion, and antioxidant capacity of LA-5. LA-5 grown with hesperidin exhibited greater resistance to pepsin and bile salt compared to LA-5 alone. Furthermore, in vitro cancer studies indicated that bacteria grown in the presence of hesperidin may increase caspase-3 activity in HT-29 cells and thus induce apoptosis in this way. This study suggests that hesperidin may enhance and contribute to the probiotic properties of LA-5. Consequently, LA-5 grown with hesperidin may exert more beneficial effects on the host.