Antimicrobial activity of crude extract of endophytic fungi isolated from leaves of Kigelia africana and Phyllanthus niruri collected from Dar es Salaam Tanzania
Effectiveness of endophytes crude extract against pathogens
Keywords:Medicinal plants; crude extracts; endophytes; ethyl acetate; n-hexane; minimum inhibition concentration
One of the main challenges that hinder achievement of the third millennium sustainable development goal is emergence of infectious agents that are resistant to existing antimicrobials. These challenges entails search for novel bioactive compounds like exploring untapped potential of medicinal plants and their associated endophytes. The present study was aimed to investigate the antimicrobial activities of crude extracts of endophytic fungi isolated from medicinal plants Kigelia africana and Phyllanthus niruri. Pure isolates of endophytic fungi were subjected to initial screening, and isolates that exhibited inhibition were genotyped by Sanger sequencing for genus and species confirmation. Then, antimicrobial activity of crude extracts of selected isolates: Nigrospora sphaerica, Meyerozyma guilliermondii, Alternaria alternata and Phyllosticta capitalensis was evaluated by disc diffusion method against E. coli (Gram-negative), S. typhi (Gram-negative), and S. aureus (Gram-positive). Findings indicated high variability (8.5 -23.1 mm) of crude extract concentrations of isolates, and the highest zone of inhibition was found in ethyl acetate crude extract of M. guilliermondii against S. aureus. Furthermore, the lowest MIC (9.9 >MIC>5.0) was found in n-hexane crude extract of P.capitalensis against S. typhi. However, all crude extracts did not show any activity against E. coli. Results of present study corroborate on the influence of extracting solvent on the effectiveness of crude extract of isolates against pathogen microorganisms. Interestingly, findings demonstrated the potential of endophytes from medicinal plants for medical applications, and therefore further investigation may lead to discovery of novel bioactive compound potent to resistant infectious agents.
Authors of articles published in Journal of BioScience and Biotechnology retain the copyright of their articles. The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.
- copyright, and other proprietary rights relating to the article, such as patent rights;
- the right to use the substance of the article in future own works, including lectures and books;
- the right to reproduce the article for own purposes, provided the copies are not offered for sale;
- the right to self-archive the article.