Application of PCR-RFLP method to determine polymorphism in BMP-15 and GDF9 fecundity genes in Northeast Bulgarian Merino sheep breed

Authors

  • Milena Bozhilova-Sakova Agricultural Academy, Institute of Animal Science – Kostinbrod, 2232 Kostinbrod, Bulgaria
  • Ivona Dimitrova University of Forestry, Faculty of Agronomy 10, Kl. Ohridski blvd., 1756 Sofia, Bulgaria

Keywords:

Northeast Bulgarian Merino sheep breed, BMP-15, GDF9, PCR-RFLP analysis, polymorphism

Abstract

The purpose of this study was to determine the presence or absence of mutations in BMP-15 and GDF9 fecundity genes. In this experiment 60 ewes from Northeast Bulgarian Merino sheep breed were tested for polymorphisms in BMP-15 gene and SNP G1 of the GDF9 gene. This is the first investigation of BMP-15 gene by PCR-RFLP method in Bulgarian sheep breed. After DNA extraction and PCR amplification were received the expected PCR products. The digestion with restriction enzyme HinfI for BMP-15 gene and HhaI for GDF0 revealed mutations in both genes. In BMP-15 were found alleles + and G with frequencies 0.94 and 0.06, respectively and genotypes ++ and G+ with frequencies 0.80 and 0.12, respectively. In GDF9 were identified alleles G and A with frequencies 0.94 and 0.06 and genotypes GG and AG with frequencies 0.88 and 0.12.

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Published

2021-12-06

How to Cite

[1]
Bozhilova-Sakova, M. and Dimitrova, I. 2021. Application of PCR-RFLP method to determine polymorphism in BMP-15 and GDF9 fecundity genes in Northeast Bulgarian Merino sheep breed. Journal of BioScience and Biotechnology. 10, 2 (Dec. 2021), 107–111.

Issue

Vol. 10 No. 2 (2021)

Section

Animal Sciences

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