Genetic diversity of Booroola gene in Northeast Bulgarian Merino sheep breed
Keywords:sheep, Northeast Bulgarian Merino breed, BMPR-1B/FecB gene, PCR-RFLP analysis
The BMPR-1B/FecB or Booroola gene is the first major fecundity gene identified in sheep. The present investigation was carried out to study polymorphism by PCR-RFLP of FecB gene in 30 ewes belonging to Northeast Bulgarian Merino sheep breed. Genomic DNA was extracted from whole blood. PCR amplification of FecB gene revealed fragments with length 190 bp using specific primer set. After PCR-RFLP analysis it was found the wild allele “+” and the mutant allele “B” with frequencies 0.97 and 0.03, respectively. This is the first study in Bulgarian sheep breeds, where was found the presence of Booroola mutation. According to the results the genotype frequencies were as followed: wild homozygous genotype “++” 0.93 and heterozygous genotype “B+” 0.07, respectively. The observed (Ho) and expected heterozygosity (He) were 0.066 and 0.058, respectively. It can be concluded that the studied herd was in Hardy-Weinberg equilibrium. PCR-RFLP technique can be used to detect and genotype FecB gene clearly.
Authors of articles published in Journal of BioScience and Biotechnology retain the copyright of their articles. The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.
- copyright, and other proprietary rights relating to the article, such as patent rights;
- the right to use the substance of the article in future own works, including lectures and books;
- the right to reproduce the article for own purposes, provided the copies are not offered for sale;
- the right to self-archive the article.