Preliminary study of Endomycodiversity among three ethnomedicinal plants family Meliaceae in Nigeria

Abstract

The natives as well as the ethnic tribes inhabiting the study area of this research work value the plants as a source of medicine to cure some of the ailments. Many of the pharmaceutical compounds produced by medicinal plants are reportedly produced by their endophytic fungi. Hence, it is important to study endophytic fungi of ethnomedicinal plants and their biodiversity.

 Modified surface sterilization techniques using 3% surfactant and 70% sterilant was applied for the explants before culturing, various biochemical tests such as wet mount using methylene blue, dalamau and urease test was employed for macro and microscopic endophytic fungal identification. Altogether 756 segments of which 26 segments each from leaves (young) and stem (twig) tissues of the respective plants was screened using modified surface sterilization techniques. Thirty one species of endophytic fungi was isolated. Based on taxonomical classification 26 belongs to Ascomycetes 2 Basidiomycetes and 5 species were recorded unidentified. The highest species richness as well as overall frequency of colonization was showed on stems of P. kotschi (20.7%) by Cladosporium species and Fusarium manilliforme from leaves (18%); Rhizoctonia species proved highest frequency of colonization on K. senegalensis stems (15.6%) followed by Cladosporium species on leaves (13.9%). Cryptococcus species showed the highest frequency of colonization on stems of A. indica (14.9%) while Cladosporium species showed the highest colonization on leaves (11%).