Sensitivity of four garden pea genotypes to physical and chemical mutagens

Authors

  • Slavka Kalapchieva Maritsa Vegetable Crops Research Institute
  • Nasya Tomlekova Maritsa Vegetable Crops Research Institute

Abstract

A study on the sensitivity of two breeding lines of garden pea to mutagenic agents was carried out in the Maritsa Vegetable Crops Research Institute, Plovdiv. The purpose was to evaluate the sensitivity of the Pisum sativum L. genotypes to physical and chemical mutagens. In the experiment, the pea seeds were irradiated single or combined with 60Co gamma rays (40, 80, 100, 200 and 400 Gy) and Ethyl methanesulfonate (EMS) at concentrations of 0.1 and 0.2%. Visible morphological changes of the stems and leaves were observed in plant of M1 generation, such as shorter stems, double petiole, clover-shaped leaves and a couple of tendrils. Single treatments with 100 Gy 60Co induced the highest mutation frequency M.F. = 7.69 for line 88-7, and М.F. = 2.11 for line 97-3, reported in M2 generation. The coefficient of interaction (k = 1.50) confirmed that the combined use of mutagens also led to an increased inducing of mutations, showing synergistic effects. Higher doses or combined gamma rays and EMS treatments induced more efficiently mutations. The results are important for increasing mutation efficiency in pea breeding.

 

Key words: gamma rays,EMS, induced mutagenesis,Pisum sativum L.

Author Biographies

Slavka Kalapchieva, Maritsa Vegetable Crops Research Institute

Breeding, Variety Maintenance and Introduction, pea breeder

Nasya Tomlekova, Maritsa Vegetable Crops Research Institute

Breeding, Variety Maintenance and Introduction,

Laboratory Head

cover

Downloads

Published

2016-09-19

How to Cite

[1]
Kalapchieva, S. and Tomlekova, N. 2016. Sensitivity of four garden pea genotypes to physical and chemical mutagens. Journal of BioScience and Biotechnology. 5, 2 (Sep. 2016), 167–171.

Issue

Vol. 5 No. 2 (2016)

Section

Genetics

License

Authors of articles published in Journal of BioScience and Biotechnology retain the copyright of their articles. The journal/publisher is not responsible for subsequent uses of the work. It is the author's responsibility to bring an infringement action if so desired by the author.

Authors retain the following rights:
  • copyright, and other proprietary rights relating to the article, such as patent rights;
  • the right to use the substance of the article in future own works, including lectures and books;
  • the right to reproduce the article for own purposes, provided the copies are not offered for sale;
  • the right to self-archive the article.