Sensitivity of four garden pea genotypes to physical and chemical mutagens
A study on the sensitivity of two breeding lines of garden pea to mutagenic agents was carried out in the Maritsa Vegetable Crops Research Institute, Plovdiv. The purpose was to evaluate the sensitivity of the Pisum sativum L. genotypes to physical and chemical mutagens. In the experiment, the pea seeds were irradiated single or combined with 60Co gamma rays (40, 80, 100, 200 and 400 Gy) and Ethyl methanesulfonate (EMS) at concentrations of 0.1 and 0.2%. Visible morphological changes of the stems and leaves were observed in plant of M1 generation, such as shorter stems, double petiole, clover-shaped leaves and a couple of tendrils. Single treatments with 100 Gy 60Co induced the highest mutation frequency M.F. = 7.69 for line 88-7, and М.F. = 2.11 for line 97-3, reported in M2 generation. The coefficient of interaction (k = 1.50) confirmed that the combined use of mutagens also led to an increased inducing of mutations, showing synergistic effects. Higher doses or combined gamma rays and EMS treatments induced more efficiently mutations. The results are important for increasing mutation efficiency in pea breeding.
Key words: gamma rays,EMS, induced mutagenesis,Pisum sativum L.
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