Induction of callogenesis and organogenesis of different melon genotypes

  • Zhana Ivanova Maritsa Vegetable Crops Research Institute, 32 Brezovsko Shosse Str., 4003 Plovdiv, Bulgaria.
  • Stanislava Grozeva Maritsa Vegetable Crops Research Institute, 32 Brezovsko Shosse Str., 4003 Plovdiv, Bulgaria.
  • Nikolay Velkov Maritsa Vegetable Crops Research Institute, 32 Brezovsko Shosse Str., 4003 Plovdiv, Bulgaria.
Keywords: Cucumis melo L., in vitro, regeneration, explant types, growth regulators

Abstract

The effect of different concentration of BA and Kinetin on regeneration of cotyledons, hypocotyls and leaves of four melon genotypes was studied. It was established that cotyledons and leaf segments were more effective as explants for bud induction and subsequent plant regeneration than hypocotyls. In the studied melon genotypes, the better regeneration was obtained in medium variants supplemented with growth regulator BA. The best response was observed in true leaves (70.0%) and cotyledons (53.3%) from line 5-1-2 in medium variant containing 1.0 mgL-1 BA. On medium variants containing Kinetin the regeneration efficiency was lower. Data from three-way analysis of variance confirmed that regeneration ability is strongly influenced by explant type and interaction of both factors - genotype and explant type. The highest number of plantlets/explant was registered in leaf segments from line K052 (0.9%), followed by cotyledons and true leaves from line 5-1-2 (0.7%) in medium variant contained 1.0 mgL-1 BA.

Published
2018-07-13
Section
Biochemistry and Biotechnology