Soybean long-term callus cultures – potential for biotransformation and nutraceutical production

Soybean long-term callus cultures


  • Georgina Petkova Kosturkova Associate professor, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia
  • Margarita S. Dimitrova
  • Rositza V. Todorova
  • Krasimira N. Tasheva
  • Peter I. Petrov
  • Shashank Tidke
  • Ravishankar A. Gokare


Soybean, Glycine max, callus, long-term cultures, biotransformation


Soybean (Glycine max (L.) Merrill.) is the world leading cultivated pulse crop as a source of protein, oil and nutraceuticals. Recently, alternative approaches for synthesis of new products gain bigger interest. Biotransformation based on iv vitro cultures is well-established technology for such alternative production. In this respect initiation of callus and long-term cultures were established from four Bulgarian soybean varieties. Potential for callus development of cotyledons and cotyledonary nodes plated on media containing various combinations of kinetin (0.1 – 1.0 mg/l) and 2,4-D (0.5 – 1.0 mg/l) was tested. The optimal concentrations for initiation and maintaining of nondifferentiated growth for most of the varieties was twice higher levels of the cytokinin to the auxin. Two of the Bulgarian genotypes (Rosa and Srebrina) revealed better potentials for callogenesis and long-term growth. These are promising for development of suitable procedures for biotransformation.





Biochemistry and Biotechnology