Antifungal activity of plant extracts against phytopathogenic fungi

Antifungal activity of plant extracts

  • Milena Tihomirova Nikolova Institute of Biodiversity and Ecosystem Research
  • Petar Yordanov AgroBioInstitute, Sofia, Bulgaria
  • Slavtcho Slavov AgroBioInstitute, Sofia, Bulgaria
  • Strahil Berkov Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria
Keywords: Artemisia, Clinopodium vulgare, exudates, GC/MS, Salvia officinalis, plant pathogens


Aqueous-methanolic extracts and acetone exudates of 24 plant species were screened for their antifungal activity against four largely spread plant pathogens possessing a broad spectrum of plant hosts - Alternaria alternata, Botrytis cinerea, Phytophthora cambivora and Fusarium oxysporum. Examination of the effect of plant extracts on mycelium growth and development of plant pathogens was done by diffusion method in vitro on PDA medium. The highest antifungal activity with an impact on the most studied pathogens was found for the acetone exudates. Among the tested exudates that of Salvia officinalis showed the highest activity against three plant pathogens tested. The exudates of Artemisia campestris, Artemisa absinthium and Clinopodium vulgare displayed activity against Fusarium oxysporum and Phytophthora cambivora. Chemical profiles of the most active exudates were determined by HPTLC and GC/MS. Non-polar substances composed mainly of flavonoid aglycones, terpenes, sterols and fatty acids were identified.

Plant Sciences